Figure 3.

AS treatment reduced PDK4 and P‐PDH expression and restored glucose oxidation in diabetic myocardium. A‐C, Cardiac PDK4 mRNA level, PDK4 protein level and p‐PDH protein level. D, Kinetic oxygen consumption rate (OCR) responses of isolated cardiomyocytes to 10 mM glucose. E, Calculated glucose oxidation rate. F, Kinetic OCR responses of isolated cardiomyocytes to 1 mM pyruvate. G, Calculated pyruvate oxidation rate. The glucose or pyruvate oxidation rate was calculated by the OCR increase (OCR value of measurement 3 subtracted by measurement 6) normalized with cell protein content. AS, AS1842856. Data are expressed as means ± SEM (n = 6). *P < .05 vs C or CCs; **P < .01 vs C or CCs; #P < .05 vs D or DCs; ##P < .01 vs D or DCs