FIGURE 4.

Jaceosidin suppressed the DMM‐induced cartilage degradation. (A‐D) Ten‐week‐old C57BL/6 mice were subjected to DMM. Mice were orally administered PBS, jaceosidin, eupatilin, both jaceosidin and eupatilin, or Seomae mugwort every other day for 10 weeks after DMM. Cartilage degradation was examined by safranin O staining (A). Scale bar = 100 μm. The severity of OA was evaluated by OARSI scores (B), osteophyte maturity (C) and subchondral bone plate thickness (D) at 10 weeks after DMM. Mice orally administered the natural compounds and the Seomae mugwort extract were compared with those administered PBS. Values are the mean ± SEM as analysed by one‐way ANOVA with Bonferroni's test (n = 10 mice per group). Significant differences from the DMM group value: **P < .01, ***P < .001, #P < .05 compared to the control group