Figure 5.

Effects of PI3K inhibitor Ly294002 on H9C2 cells exposed to Rg1 and/or G&P. H9C2 cells were pre‐treated with Rg1 (40 μmol/L) with or without Ly294002 (10 μmol/L) for 2 h, and then exposed to G&P with 40 μmol/L Rg1 treatment with or without Ly294002 (10 μmol/L). A, Cell viability in different treatment groups (control; Glu + Pal; Rg1 + Glu +Pal; Ly294002 + Rg1 + Glu + Pal); n = 5 per group. B, LDH release in culture medium in each group; n = 5 per group. C, Cleaved caspase‐3 expression in each group tested by Western blotting; n = 5 per group. *P < .05 vs control group, ^# P < .05 vs Glu + Pal group, ^& P < .05 vs Rg1 + Glu +Pal group