Figure 6.

AKT/GSK‐3β/Nrf2 pathway plays an important role in Rg1 protection against G&P‐induced H9C2 injury. H9C2 cells were pre‐treated with Ly294002 (10 μmol/L) for 2 h, then co‐treated with Rg1 (40 μmol/L) for another 24 h. A, Expression of p‐AKT, t‐AKT, p‐GSK‐3β, t‐GSK‐3β in different groups (control; Rg1; Ly294002; Ly294002 + Rg1) tested by Western blotting; n = 5 per group. B, Nrf2, HO‐1, and NQO‐1 expression in each group (control; Rg1; Ly294002; Ly294002 + Rg1) tested by Western blotting; n = 5 per group. *P < .05 vs control group, #P < .05 vs Rg1 group