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. 2020 May 23;24(14):7814–7828. doi: 10.1111/jcmm.15412

FIGURE 7.

FIGURE 7

ASK1 mediates the function of ARRB1 during liver I/R injury. ASK1 inhibitor NQDI‐1 or DMSO (control group) was injected with ARRB1 KO and WT mice before I/R operation. A, Western blot was conducted to detect the expression levels of p‐ASK1, ASK1, p‐JNK1/2, p‐p38 and p‐p65 in ARRB1 KO and WT mice pretreated with NQDI‐1 or DMSO after liver I/R injury for 12 h. B, Representative images and analysis of histological H&E staining showing necrotic areas in livers from ARRB1 KO or WT mice pretreated with NQDI‐1 or DMSO and subjected to hepatic I/R for 12 h (magnification, ×200, n = 5‐6 per group). C, The activities of serum AST and ALT and (D) pro‐inflammatory cytokines in the indicated group (n = 5‐6 per group). E, LDH release of primary hepatocytes isolated from ARRB1 KO and WT mice injected with NQDI‐1 or DMSO. *P < .05; all data are presented as mean ± SD; significance determined by one‐way ANOVA