FIG 7.

Overexpression of gtsE slows the growth of B. anthracis. (A) Growth curves of strain Sterne carrying empty vector pJK4 or plasmids harboring galE1, gtsA, gtsB, gtsC, and gtsE grown in BHI broth with IPTG at 37°C were obtained by monitoring the absorbance of cultures over time at 600 nm. Bars indicate standard deviations (n = 3). (B) Vegetative bacillus variants were prepared and stained with monoclonal antibody EAII6G6-FITC or lectin ligands as described in the legend to Fig. 3. Representative bright-field (BF) and fluorescence microscopy images are shown. α and β indicate the configuration of ligands for lectins and antibodies.