TABLE 1.
Bioinformatic analysis of key glycosyltransferases identified in this studya
| Gene | Protein name | Length (amino acids) | Classification or predicted family | Predicted DXD-like motif(s) | Predicted activity | No. of TM segments | Predicted localization of GT domain |
|---|---|---|---|---|---|---|---|
| bas5287 | GtsA | 323 | Dolichol-phosphate-mannose synthetaseb | 94DAD | GT | 2 | Cytoplasmic |
| bas5286 | GtsB | 127 | GtrA family; PF04138 | No | Flippase | 4 | NA |
| bas5285 | GtsC | 479 | GT family C fold; PF13231 | Unknown | GT | 12 | Extracytoplasmic |
| bas5280 | GtsE | 273 | GT family A fold; PF00535 | 86DDD, 162DED | GT | 0 | Cytoplasmic |
a
Functional and topology predictions were obtained using InterPro and TMHMM analyses. TM, transmembrane; GT, glycosyltransferase; NA, not applicable.
b
This family of glycosyltransferases has been shown to transfer the sugar moiety from carriers such as UDP‐glucose, UDP‐N‐acetylgalactosamine, GDP‐mannose, or TDP‐rhamnose onto substrates that include cellulose, dolichol phosphate, and teichoic acid.