TABLE 3.
MALDI-TOF mass spectrometry of hydrofluoric acid-released SCWPs from B. anthracis variants lacking one or more predicted glycosyltransferasesa
| Proposed composition of SCWPb | Observed m/z in strain with genotype: |
Theoretical m/z (monoisotopic) | ||||
|---|---|---|---|---|---|---|
| ΔgtsD1::aad9 | ΔgtsC-gtsD1::aad9 ΔgtsE | ΔgtsC-gtsD1::aad9 ΔgtsE/pspac-gtsC | ΔgtsA::aad9 ΔgtsE | ΔgtsA::aad9 ΔgtsE/pspac-gtsA | ||
| HexNAc3Na+ | 649.85 | 649.82 | 649.80 | 649.81 | 649.83 | 649.97 |
| HexNAc3-Gal2Na+ | 976.34 | 974.26 | ||||
| HexNAc3-Gal3Na+ | 1,136.31 | 1,136.40 | ||||
| HexNAc4-Gal2Na+ | 1,175.30 | 1,175.40 | 1,177.45 | |||
| HexNAc3-GlcN-Gal4Na+ | 1,459.81 | 1,459.52 | ||||
| HexNAc5-GlcN-Gal32Na+ | 1,725.53 | 1,724.53 | 1,725.86 | |||
| HexNAc6-Gal4Na+ | 1,908.43 | 1,907.70 | 1,908.13 | |||
| HexNAc6-GlcN-Gal42Na+ | 2,090.60 | 2,091.63 | 2,092.11 | |||
| HexNAc9-Gal6Na+ | 2,841.73 | 2,842.00 | ||||
| HexNAc9-Gal9Na+ | 3,327.78 | 3,327.18 | ||||
| HexNAc12-Gal12Na+ | 4,424.01 | 4,422.57 | ||||
a
Ion signals and proposed compositions of compounds from MALDI-TOF mass spectra of RP-HPLC-purified SCWPs from various strains.
b
Additional compositional explanations for the observed masses exist but are not listed here. HexNAc, ManNAc (N-acetylmannosaminyl) and GlcNAc (N-acetylglucosaminyl); GlcN, glucosaminyl; Gal, galactosyl.