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. Author manuscript; available in PMC: 2020 Jul 10.
Published in final edited form as: Neuron. 2014 Sep 18;84(1):123–136. doi: 10.1016/j.neuron.2014.08.056

Figure 2. GINIP Defines a Subset of Nonpeptidergic Nociceptors that Targets Lamina II.

Figure 2.

(A) Characterization of GINIP-positive population in DRGs. Double immunostaining using rat anti-GINIP antibody associated with rabbit anti-TrkA or goat anti-cRet or goat anti-TrkC antibodies or Alexa 565-conjugated isolectin-B4. In situ hybridization using antisense probes for ginip, trkB, or gfrα2 followed by immunostaining using rat anti-GINIP antibody. Double in situ hybridization using mrgprD and tafa4 antisense probes followed by immunostaining using rat anti-GINIP antibody. The GINIP population can be divided into two subpopulations: mrgprD+ and tafa4+ neurons. Scale bar, 100 μm.

(B) Triple immunostaining using rat anti-GINIP and rabbit anti-PKCγ antibodies in combination with Alexa 488-conjugated isolectin-B4 on spinal cord sections.

(C) Triple immunostaining using rat anti-GINIP, rabbit anti-CGRP antibodies, and Alexa 488-conjugated isolectin-B4. Colabeled GINIP/IB4 fibers innervate lamina IIo. GINIP+/IB4− fibers target lamina IIi PKCγ+ interneurons. Lamina I (CGRP staining) is devoid of GINIP staining. Scale bar, 100 μm. See also Figure S1.