FIGURE 4.

Representative images illustrating the CLSM in vivo detection of H₂S (red color) and peroxisomes (green color) in root tips (A–C) and guard cells (D–G) of 10 days old Arabidopsis seedlings expressing CFP-PTS1. (A,D) Fluorescence puncta (green) attributable to CFP-PTS1, indicating the localization of peroxisomes. (B,E) Fluorescence punctate (red) attributable to H₂S detection in the same area. (C) Merged image of (A,B) showing colocalized fluorescence punctate (yellow). (F) Chlorophyll autofluorescence (blue) demonstrating location of chloroplasts. (G) Merged images of (D–F). H₂S (red color) was detected by using 5 mM WSP-5, a fluorescence probe for H₂S. Arrows indicate representative punctate spots corresponding to the colocalization of H₂S with peroxisomes. Asterisks indicate localization of H₂S in the cytosol. Reproduced with permission from Corpas et al. (2019a) provided by John Wiley and Sons.