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. 2020 Jul 3;11:899. doi: 10.3389/fpls.2020.00899

TABLE 2.

Segregation ratios for ECD 02 × CR 1505 derived F2 population for resistance to clubroot under greenhouse conditions.

Popl/Fam Pathotypeφ Total no. of F2 (Score = no. plants) Tested ratio DF Observed (disease score)1
Test of statistics1
Observed (disease score)2
Test of statistics2
R (0) S (1 + 2 + 3) χ2 Prob R (0 + 1) S (2 + 3) χ2 Prob
Popl#2 Fam 1 3H (SACAN-ss1) 227
(Score 0 = 104)
(Score 1 = 26)
(Score 2 = 20)
(Score 3 = 77)
3R:1S 1 104 123 103.1 <0.00001 130 97 38.1 <0.00001
1R:3S 1 52.5 <0.00001 126.1 <0.00001
9R:7S 1 10.0 0.0015 0.1 0.7570*
7R:9S 1 0.4 0.5306* 16.9 0.00004
5R:11S 1 22.4 <0.00001 71.5 <0.00001
11R:5S 1 55.6 <0.00001 13.9 0.0002
13R:3S 1 187.1 <0.00001 85.7 <0.00001
3R:13S 1 109.1 <0.00001 221.1 <0.00001
15R:1S 1 890.2 <0.00001 515.6 <0.00001
1R:15S 1 606.5 <0.00001 1008.4 <0.00001
63R:1S 1 4086.8 <0.00001 2501.4 <0.00001
1R:63S 1 2890.2 <0.00001 4579.9 <0.00001
Popl#2Fam 1 5X(LG-1) 244
(Score 0 = 143)
(Score 1 = 35)
(Score 2 = 12)
(Score 3 = 54)
3R:1S 1 143 101 35.0 <0.00001 178 66 0.5 0.4598*
1R:3S 1 147.0 <0.00001 299.2 <0.00001
9R:7S 1 0.6 0.4581* 27.7 <0.00001
7R:9S 1 21.9 <0.00001 84.5 <0.00001
5R:11S 1 85.0 <0.00001 197.5 <0.00001
11R:5S 1 11.7 0.0006 2.0 0.1569*
13R:3S 1 82.1 <0.00001 11.0 0.0009
3R:13S 1 254.4 <0.00001 470.5 <0.00001
15R:1S 1 514.3 <0.00001 180.1 <0.00001
1R:15S 1 1141.5 <0.00001 1852.7 <0.00001
63R:1S 1 2516.8 <0.00001 1030.5 <0.00001
1R:63S 1 5162.1 <0.00001 8084.7 <0.00001
Popl#2Fam 1 5G(CDCS) 239
(Score 0 = 137)
(Score 1 = 25)
(Score 2 = 16)
(Score 3 = 61)
3R:1S 1 137 102 39.8 <0.00001 162 77 6.6 0.0010
1R:3S 1 133.2 <0.00001 233.3 <0.00001
9R:7S 1 0.1 0.7383* 12.9 0.0003
7R:9S 1 17.9 <0.00001 56.1 <0.00001
5R:11S 1 75.6 <0.00001 148.5 <0.00001
11R:5S 1 14.5 0.0001 0.1 0.7469*
13R:3S 1 89.8 <0.00001 28.5 <0.00001
3R:13S 1 233.4 <0.00001 377.2 <0.00001
15R:1S 1 541.3 <0.00001 275.0 <0.00001
1R:15S 1 1063.9 <0.00001 1544.4 <0.00001
63R:1S 1 2626.8 <0.00001 1460.2 <0.00001
1R:63S 1 4831.2 <0.00001 6813.9 <0.00001

*Observed segregation ratio not significantly different from the tested ratio at P ≤ 0.05. ϕPathotype 3H (SACAN-ss1) is a single spore isolate (Xue et al., 2008) while isolates represented by pathotypes 5X (LG-1) and 5G (CDCS) are field isolates (Strelkov et al., 2016, 2018). 1Plants with disease score = 0 as R and those with disease scores 1, 2 and 3 as S. 2Plants with disease scores = 0 and 1 as R and those with disease scores 2 and 3 as S.