Figure 1.

Paper workflow. (A) in-silico-based target selection for miR-34a, including protein interaction analysis by STRING. Putative miR-34a target genes were predicted by an in-silico-based approach and computationally filtered for IRE1α-mediated unfolded protein response resulting in the three target genes BIP, IRE1α and XBP1. STRING analysis: magenta lines: experimentally determined protein interactions; blue lines: known interactions from curated databases; yellow lines: text mining. (B) experimental setup. The target genes BIP, IRE1α and XBP1 for miR-34a-5p were experimentally validated, and the effect of miR-34a targeting was analyzed by functional analysis.