Skip to main content
. 2020 Jun 18;9(6):1484. doi: 10.3390/cells9061484

Figure 3.

Figure 3

RO4929097 inhibited gliotoxin-induced and basal NOTCH3 mRNA expression in CLL cells. (A) Time kinetic RT-PCR analysis showing NOTCH1, NOTCH2, and NOTCH3 mRNA expression in response to 0.2 µM gliotoxin and/or 0.5 µM RO4929097 treatment in relation to the GSI sensitivity of nuclear NOTCH2. For co-treatment, CLL cells were first incubated with RO4929097 immediately before adding gliotoxin. The effect of the compounds on the percentage of apoptotic CLL cells after 1 day in culture is indicated. (B) FACS analysis indicating the inhibition/induction of apoptosis by RO4929097 (0.5 µM) in fresh CLL samples derived from two representative NOTCH2 GSI-R Rai/Binet I/A and two representative NOTCH2 GSI-S Rai/Binet IV/C CLL patient samples after 7 days in culture. (C) Corresponding RT-PCR showing the effect of RO4929097 (0.5 µM) on NOTCH1, NOTCH2, and recovered NOTCH3 mRNA expression on Day 7. The GSI resistance/sensitivity of nuclear NOTCH2/CSL DNA-complexes is indicated by EMSA. ACTB was included as the internal control.