Skip to main content
. 2020 Jun 18;11(6):663. doi: 10.3390/genes11060663

Figure 3.

Figure 3

Dynamics of expression of genes associated with dispersed alpha satellite repeats after HS. Expression of: (A) ZNF675 associated with polymorphic alpha satellite repeat 29 which is present in cell lines MJ90 hTERT and HepG2, and absent in Cal 27 and OV-90, under standard conditions (no HS), immediately after 2 h of HS (0 min) and 30 min of recovery, (B) NR3C1 in cell lines MJ90 hTERT, Cal 27 and OV-90 where alpha repeat 10 is present on both alleles, and (C) NR3C1 allele specific expression in HepG2 cells from the allele with (HepG2+) and without (HepG2-) alpha repeat 10, under standard conditions (no HS), after 2 h of heat stress (0 min), and at 30 min and 1 h of recovery, (D) SLC30A6, PRIM2, STAM, and MYO1E with alpha repeats in intron and SLC40A1, ASNSD1, ST6GAL1, HTRA3, ACOX3, INTS1, PHF20L1, DIP2C associated with intergenic alpha repeats. Expression is analyzed in MJ90 hTERT cell line under standard conditions (no HS) and immediately after 2 h of HS (0 min). In (A–C) No represents normalized average No value for each gene. In (D) relative No values are shown that are obtained by dividing each No value by No value of control (no HS), for each gene. Columns show average of two different RT-qPCR experiments performed in triplicate and error bars represent standard deviations.