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. 2020 May 29;9(6):1353. doi: 10.3390/cells9061353

Figure 3.

Figure 3

Typical trends of H2, O2 and acetic acid concentrations of Chlamydomonas cultures incubated in TAP (A) and TAP-S (B). In TAP cultures (A), H2 production occurs only in the presence of acetic acid, which is necessary to establish hypoxic conditions. In TAP-S cultures (B), the H2 production phase and the hypoxic phase are independent of the acetic acid concentration. Under the same light conditions, TAP cultures show faster acetic acid uptake and shorter lag phase than in TAP-S. H2 production yield and duration in TAP-S cultures is often higher than in TAP cultures. Tlag, lag phase before H2 production; tp, H2 production phase; thypoxia, hypoxia/anaerobic phase; (O2)in, initial O2 levels; (O2)hyp, minimal O2 levels compatible with H2 production.