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. 2020 May 26;9(6):1333. doi: 10.3390/cells9061333

Figure 4.

Figure 4

Cytotoxic effect of 3-bromopyruvate (3-BP) on GIST cells (A) Giemsa staining shows the number of adherent cells upon treatment with 3-BP (100 µM, 8 h) or dimethyl sulfoxide (DMSO). (B) The viability of GIST cells treated with 3-BP was measured by the WST-1 assay. Histograms represent mean ± SD. ** p < 0.01, *** p < 0.001 (Student’s t-test). (C) Representative immunoblots of full length and cleaved poly (ADP-ribose) polymerase (PARP) in the GIST 882 and 882R cells treated with 3-BP or DMSO. (D) Quantification of cleaved PARP/β-actin ratio from three independent biological experiments. (E) Representative flow cytometric images of the effect of 3-BP treatment on cell apoptosis by AnnexinV-APC and propidium iodide (PI) staining. The early and late apoptotic cells are presented by Annexin V+/PI− (lower right quadrant, Q1) and Annexin V+/PI+ (upper right quadrant, Q2) stained cells, respectively. (F) Quantification of the apoptotic cells from three independent biological experiments. Histograms represent mean ± SD. *** p < 0.001, (Student’s t-test).