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. 2020 Mar 25;8(2):145. doi: 10.3390/vaccines8020145

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression of recombinant AgBR1 protein and polyhistidine-tag cleavage. Recombinant AgBR1 protein was expressed using stable transfected S2 cells, and the polyhistidine tag was cleaved by TEV protease. (a) Analysis of purified recombinant AgBR1-TEV-V5-His and His-cleaved AgBR1 protein by SDS-PAGE stained with Coomassie Brilliant Blue (CBB). (b) Analysis of AgBR1 His-cleaved recognition by rabbit anti-AgBR1 specific antibodies by Western Blotting. (c) Analysis of His-cleavage efficacy by Western Blotting using an anti-His tag specific antibody.