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. 2020 Jun 3;10(6):344. doi: 10.3390/brainsci10060344

Figure 4.

Figure 4

Characterization of synaptic varicosities on D50 iDNs. iDNs were labeled with a synapsin antibody costained for phalloidin 488 (green) indicating F-actin+ axonal structures. Magnifications highlight individual synaptic varicosities, putative axo-axonal synapses (red) both in regions of distal (A) and proximal neurites (B). 2–3 individual differentiations were used, bar 20 µm, 1 µm (enlargement), line scan was set to 3 µm.