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. 2020 Apr 13;8(2):179. doi: 10.3390/vaccines8020179

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunization with combinations of HCMV gB, HCMV gH/gL and/or UL128/UL130/UL131A proteins induced strong synergistic neutralizing activities preventing HCMV infection of epithelial cells. Day 72 immune sera from rabbits (n = 5) subcutaneously immunized three times with 25 µg of trimeric HCMV gB, HCMV gH/gL and UL128/UL130/UL131A individually or in various combinations (25 µg each) adjuvanted with alum + CpG-ODN were either heat-inactivated (A) or non-heated (B). IC₅₀ neutralizing activities were determined using HCMV strain AD169^wt131 and the ARPE-19 epithelial cell line. Significance #, $, * p < 0.05 compared to the sum of HCMV neutralizing activities of the sera from rabbits immunized with individual envelope proteins.

Immunization with combinations of HCMV gB, HCMV gH/gL and/or UL128/UL130/UL131A proteins induced strong synergistic neutralizing activities preventing HCMV infection of epithelial cells. Day 72 immune sera from rabbits (n = 5) subcutaneously immunized three times with 25 µg of trimeric HCMV gB, HCMV gH/gL and UL128/UL130/UL131A individually or in various combinations (25 µg each) adjuvanted with alum + CpG-ODN were either heat-inactivated (A) or non-heated (B). IC₅₀ neutralizing activities were determined using HCMV strain AD169^wt131 and the ARPE-19 epithelial cell line. Significance #, $, * p < 0.05 compared to the sum of HCMV neutralizing activities of the sera from rabbits immunized with individual envelope proteins.