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. 2020 Apr 19;8(2):190. doi: 10.3390/vaccines8020190

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Antibody-secreting cell (ASC) response in NLP-CSP_CT-immunized mice. Groups of four C57BL/6 mice were immunized with three doses of NLP-CSP_CT conjugated with Poly (I:C) adjuvant. The antigen-specific ASC response in the spleen was measured on days 0 and 5, and in the bone marrow on days 0 and 30 after the last dose. A representative example of the ASC response specific to yPvCSP-All_CT, NLP-CSP_CT, FliC-VK210, FliC-VK247, and FliC-P. vivax-like in the spleen is shown in (a), while that of the bone marrow is shown in (c). The wells shown in the figure were plated with 0.5 × 10⁵ cells. The total ASCs were measured for IgG secreting cells specific to the above-mentioned proteins. The data points represent the average ASC counts observed per million cells, while the bars represent the ± SEM (b and d) and frequency (e and f). The results were statistically compared using one-way ANOVA followed by Tukey’s test. Significant differences between groups are denoted on the graph: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. Non-significant (ns) differences are indicated (p > 0.05). The experiment was performed twice with four mice per group.

Antibody-secreting cell (ASC) response in NLP-CSP_CT-immunized mice. Groups of four C57BL/6 mice were immunized with three doses of NLP-CSP_CT conjugated with Poly (I:C) adjuvant. The antigen-specific ASC response in the spleen was measured on days 0 and 5, and in the bone marrow on days 0 and 30 after the last dose. A representative example of the ASC response specific to yPvCSP-All_CT, NLP-CSP_CT, FliC-VK210, FliC-VK247, and FliC-P. vivax-like in the spleen is shown in (a), while that of the bone marrow is shown in (c). The wells shown in the figure were plated with 0.5 × 10⁵ cells. The total ASCs were measured for IgG secreting cells specific to the above-mentioned proteins. The data points represent the average ASC counts observed per million cells, while the bars represent the ± SEM (b and d) and frequency (e and f). The results were statistically compared using one-way ANOVA followed by Tukey’s test. Significant differences between groups are denoted on the graph: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. Non-significant (ns) differences are indicated (p > 0.05). The experiment was performed twice with four mice per group.