Figure 6.
Evaluation of parasitemia after challenge in mice immunized with a prime-boost regimen. (a) C57BL/6 mice were immunized s.c. with Poly (I:C) or a mixture of Poly (I:C) and 10 µg of recombinant protein, NLP-CSPCT. On day 58 after priming, the mice were challenged with 5000 Pb/PvVK210 transgenic sporozoites. (b) Surveillance curve with the percentage of non-infected mice in each experimental group is shown. (c) Parasitemia was analyzed by flow cytometry. The percentage of infected red blood cells (iRBCs) on days 4, 5, and 6 post-infection (p.i.) was expressed as log values for normalization before statistical analysis. (d) The log of parasitemia on day 5 (D5) post-challenge was measured in mice in each of the immunized groups. Data from two independent experiments and significance were determined by two-tailed unpaired t-test (Mann–Whitney test).