Figure 5.

Percentages of antigen-specific CD4⁺ or CD8⁺ T-cells. Splenic (A) CD4⁺ T cells, and (B) CD8⁺ T-cells 2 weeks after two intramuscular immunizations spaced 4 weeks apart in BALB/c mice. Mice were immunized with either 1.5 or 0.15 μg/dose of self-amplifying RNA expressing rabies G glycoprotein adjuvanted with either polymeric nanoparticles (NPs), DOTAP liposomes or DDA liposomes, and spleens from 3 mice randomly selected out of the group were tested. Candidates were compared with the commercial vaccine Rabipur (1/20 of human dose) and GSK trademark CNE56 as positive controls. Splenocytes were stimulated with rabies G1/G2/G3 peptide, stained for intra-cellular cytokines, and analyzed by flow cytometry. The color code indicates the different combinations of cytokine produced by the respective cells. Unstimulated cells were used as control. Refer to Figure S3 in the Supplemental Material for the gating strategy.