. 2020 May 8;8(2):212. doi: 10.3390/vaccines8020212

Table 2.

Schematic representation of criteria applied to DOTAP- or DDA-based liposomes, solid lipid nanoparticles and emulsions to down-select formulations to progress to in vivo studies.

			Progression Selection Criteria
Cationic Lipid	Delivery Platform	Method of Association	Progress to In Vitro Based on Physio-Chemical Attributes	Progress to In Vitro Based on In Vitro Efficacy
DOTAP	Liposomes	Adsorbed	✓	✕
	Liposomes	Entrapped	✓	✓
	SLNs	Adsorbed	✓	✕
	SLNs	Entrapped	✓	✕
	NPs	Adsorbed	✕	✕
	NPs	Entrapped	✓	✓
	Emulsions	Adsorbed	✓	✕
DDA	Liposomes	Adsorbed	✓	✕
	Liposomes	Entrapped	✓	✓
	SLNs	Adsorbed	✓	✕
	SLNs	Entrapped	✕	✕
	NPs	Adsorbed	✓	✕
	NPs	Entrapped	✕	✕
	Emulsions	Adsorbed	✓	✕