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. 2020 May 26;9(6):1327. doi: 10.3390/cells9061327

Figure 8.

Figure 8

Targeting mitogen activated protein kinase kinase 3 (Map2k3) by miR-690 reduces downstream inflammation and cell stress. (A) Western blot image of protein analysis. miR-690 mimic significantly reduced the amount of phospho-p38 (P-p38) and phospho-p65 (P-p65) at protein level compared to negative control (NC), whereas inhibitor-treated groups had no differences in the protein amount compared to NC. (B) Quantification of P-p38 protein normalized to Tubulin. (C) Graphical interpretation of P-p65 protein level normalized to Tubulin level. (D) Mapk14/p38 did not show any significant reduction in messenger RNA (mRNA) level with miR-690 mimic or inhibitor treatments compared to NC. (E) Similar to p38, p65 showed no changes at mRNA levels among treatment groups. (F) Enzyme-linked immunosorbent assay (ELISA) results. miR-690 mimic significantly reduced interleukin 6 (IL-6) level in media treated with miR-690 mimic compared to NC. Common letters on the error bars indicate no significance (e.g., “a” is significantly different from “b” and “ab” indicates no significance compared to “a” and “b”). Data are presented as mean ± SEM (p < 0.05) (n = 4).