Figure 1.

Construction of recombinant Newcastle disease virus (NDV) expressing peste des petits ruminants virus (PPRV) hemagglutinin (H). Schematic representation of genomic organization of parental rNDV and newly generated rNDV_H_Kur expressing PPRV hemagglutinin (H). The PPRV transgene is inserted between the genes encoding the NDV fusion protein (F) and the hemagglutinin-neuraminidase (HN). The open reading frame (orf) is flanked by the gene start sequence (GS, grey triangle) and the gene end sequence (GE, grey rectangle), and as indicated by the NDV HN-non-coding regions (ncr, white rectangles). The basis of virus rescue is the full-length plasmids pNDVGu und pNDV_H_Kur. The 6035 bp NotI-BsiWI fragment of pUCNDV_H_Kur was inserted into pNDVGu after cleavage of the respective plasmids with NotI and BsiWI, resulting in pNDV_H_Kur.