Figure 3.
In vitro replication in cell lines originated from different species. (A) Chicken embryo fibroblasts (CEFs), (B) ovine fetal thymus cells (SFT-R), or (C) caprine tongue mucosa cells (ZZ-R) were infected with rNDV or rNDV_HKur (moi 0.01). Cell culture supernatants were harvested at indicated time points after infection (p.i.) and titrated on quail muscle (QM9) cells. Viral titers (TCID50/mL) were determined by subsequent immunostaining using a hyperimmune serum against NDV and Alexa 488 α-rabbit secondary antibody (Invitrogen). Bar charts depict mean viral titers ± standard deviation (n = 4, two samples each from two independent experiments).