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. 2020 Apr 28;8(2):205. doi: 10.3390/vaccines8020205

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Virus dissemination and shedding after PPRV challenge infection. Five goats each were either left unvaccinated (group 1), double inoculated with rNDV (group 2), single vaccinated with PPRV Nigeria 75/1 (group 3), single vaccinated with rNDV_H_Kur (group 4), or double vaccinated with rNDV_H_Kur (group 5). All goats were infected with PPRV Kurdistan/11 three weeks after immunization. (A) Blood, (B) nasal swabs, (C) ocular swabs, (D) oral swabs, and (E) fecal swabs were taken at indicated days after challenge infection (dpc) and analyzed by quantitative real-time RT-PCR (RT-qPCR) for the presence of PPRV N specific RNA. Values were transformed to genome equivalents (GEQ) using calibration curves of defined RNA standards that were included within each RT-qPCR run. The number of positive samples relating to the total number of samples tested by RT-qPCR is given below the boxplot. Asterisks (*) and numbers above datasets indicate significant differences (α = 0.05) between respective groups at the same day.

Virus dissemination and shedding after PPRV challenge infection. Five goats each were either left unvaccinated (group 1), double inoculated with rNDV (group 2), single vaccinated with PPRV Nigeria 75/1 (group 3), single vaccinated with rNDV_H_Kur (group 4), or double vaccinated with rNDV_H_Kur (group 5). All goats were infected with PPRV Kurdistan/11 three weeks after immunization. (A) Blood, (B) nasal swabs, (C) ocular swabs, (D) oral swabs, and (E) fecal swabs were taken at indicated days after challenge infection (dpc) and analyzed by quantitative real-time RT-PCR (RT-qPCR) for the presence of PPRV N specific RNA. Values were transformed to genome equivalents (GEQ) using calibration curves of defined RNA standards that were included within each RT-qPCR run. The number of positive samples relating to the total number of samples tested by RT-qPCR is given below the boxplot. Asterisks (*) and numbers above datasets indicate significant differences (α = 0.05) between respective groups at the same day.