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. 2020 Apr 28;8(2):205. doi: 10.3390/vaccines8020205

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Serology after PPRV challenge infection. Five goats each were either left unvaccinated (group 1), double inoculated with rNDV (group 2), single vaccinated with PPRV Nigeria 75/1 (group 3), single vaccinated with rNDV_H_Kur (group 4), or double vaccinated with rNDV_H_Kur (group 5). All goats were infected with PPRV Kurdistan/11 three weeks after immunization. (A) Sera were taken at indicated days after challenge infection (dpc) and analyzed for the presence of antibodies, specific to PPRV N, by competitive ELISA (cELISA). A dotted line indicates the respective thresholds. The number of seropositive tested samples, relating to the total number of tested samples, is given below the boxplots. (B) Sera from goats, vaccinated with Nigeria 75/1 or rNDV_H_Kur, were tested for the presence of neutralizing antibodies against PPRV Nigeria 75/1 by virus neutralization test (VNT). Sera exhibiting a VNT-titer >10 are considered seropositive (dotted line), sera can reach a maximum titer of 640, as they were not further titrated (dotted line).