Figure 9.
Analyses of postmortem organ samples. Five goats each were either left unvaccinated (group 1), double inoculated with rNDV (group 2), single vaccinated with PPRV Nigeria 75/1 (group 3), single vaccinated with rNDV_HKur (group 4), or double vaccinated with rNDV_HKur (group 5). All goats were infected with PPRV Kurdistan/11 three weeks after immunization. Goats from group 1 and 2 were euthanized on day 9 post challenge infection (dpc), except for two animals from group 1, which were euthanized 14 dpc. All goats from group 3, 4 and 5 were euthanized 28 dpc. Samples from lung, spleen, liver, and mediastinal and mesenterial lymph nodes were analyzed by quantitative real-time RT-PCR (RT-qPCR) for the presence of PPRV N specific RNA. Values were transformed to genome equivalents (GEQ) using calibration curves of defined RNA standards that were included within each RT-qPCR run. Boxplots also depict individual values for a better overview. The number of positive samples relating to the total number of samples tested by RT-qPCR is given below the boxplot. Asterisks (*) and numbers above datasets indicate significant differences (α = 0.05) between respective groups at the same day.