Table 1.
Molecular primers and cycling conditions used to test samples.
| Virus | Primer | Primer Sequence | Cycling Conditions | |||
|---|---|---|---|---|---|---|
| Alphavirus | Vir2052F | TGG CGC TAT GAT GAA ATC TGG AAT GTT | 95 °C (10 min) |
95 °C (30 s) 49 °C (30 s) 72 °C (30 s) for 35 cycles |
72 °C (10 min) |
4 °C (hold) |
| Vir2052R | TAC GAT GTT GTC GTC GCC GAT GAA | |||||
| Flavivirus | Fu1 | TAC AAC ATG ATG GGA AAG AGA GAG AA | 95 °C (10 min) |
95 °C (30 s) 55 °C (30 s) 68 °C (45 s) for 35 cycles |
72 °C (7 min) |
4 °C (hold) |
| CDF2 | GTG TCC CAG CCG GCG GTG TCA TCA GC | |||||
| Ortho-bunyavirus | BCS82c | ATG ACT GAG TTG GAG TTT CAT GAT GTC GC | ||||
| BCS332v | TGT TCC TGT TGC CAG GAA AAT | |||||