FIGURE 4.

Effects of deleting predicted cre and CodY‐box on CodY binding to the cid and lrg promoter regions. Underlined and letters indicate putative CcpA‐binding (cre1 and cre2) and CodY‐binding (CodY1 and CodY2) sequences in the cid (a) and lrg (b) promoter regions, respectively. EMSAs were performed with 1 fmol biotinylated cid (Pcid) and lrg (Plrg) regions with a deletion in the putative cre and CodY‐box sequences and various amounts of purified His‐CodY. Each binding reaction mixture contains 0, 6.25, or 12.5 pmol of His‐CodY protein. (a) Lanes 1–3 contain biotinylated Pcid wild‐type DNA probes; lanes 4–6 contain biotinylated cre1 deleted DNA probes; and lanes 7–9 contain biotinylated cre2 deleted DNA probes. (b) Lanes 1–3 contain biotinylated Plrg wild‐type DNA probes; lanes 4–6 contain biotinylated CodY1 deleted DNA probes; and lanes 7–9 contain biotinylated CodY2 deleted DNA probes. The reactions were run on a non‐denaturing polyacrylamide gel and the signal observed via chemiluminescence. Presented results are representative of two independent experiments