Figure 1.

Sequence of SPARC promoter region (−29 bp to +191 bp) located spanning from exon 1 to intron 1 of the gene. Eleven CpGs are marked as red triangle shape and numbers are given from 5′ to 3′ of positive strand of the SPARC gene. The QMSP amplified region counts 111 bp (Forward in purple arrow, Probe in green segment and Reverse in red arrow). The letters colored and underlined indicate SP1 (blue) and AP1 (red) binding consensus sequences, respectively. * indicates the CpGs included in the 450 K array.