Skip to main content
. 2020 Jun 22;9(6):1523. doi: 10.3390/cells9061523

Figure 4.

Figure 4

(A) Representative QMSP analysis showing the comparison between the promoter methylation levels of SPARC in NSCLC cell lines (A549, H2228, H1573, H460). Two normal lung cell lines NL20 and MRC-5 were used as negative controls. Changes in (B) SPARC promoter methylation levels detected by QMSP and (C) SPARC mRNA transcript levels by quantitative real-time (RT-qPCR) in the A549 cell line before and after treatment with 5µm of 5-azacytidine (5-aza-Dc, AZA) at 24 (AZA 24 h) and 48 h (AZA 48 h). Error bars indicate the standard deviation of three different experiments. * p < 0.05, *** p < 0.001 (t-test).