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. 2020 Jun 1;9(6):1369. doi: 10.3390/cells9061369

Figure 2.

Figure 2

Resistin stimulates VCAM-1 expression and monocyte adhesion to osteoarthritis synovial fibroblasts (OASFs). (A–D) OASFs were incubated with resistin (0, 1, 3, and 10 ng/mL) only or resistin at 10 ng/mL + VCAM-1 antibody for 24 h. THP-1 cells were subsequently added to OASFs for 1 h. The adherence of THP-1 cells to cultured OASFs was photographed under fluorescence microscopy (n = 4) (A) and quantified (B). VCAM-1 expression according to varying concentrations of resistin (0, 1, 3, or 10 ng/mL) was quantified by Western blotting (n = 3) (C) and RT-qPCR analysis (n = 4) (D). (E) IHC staining of VCAM-1 for normal (n = 10) and OA synovium (n = 10). ** p < 0.01; *** p < 0.001 compared with the control group; ### p < 0.001 compared with the resistin-treated group.