Figure 3.

Application of different serum and plasma samples on adult human cardiac stem cells. (A) hCSCs were exposed to blood serum and blood plasma from old (>60 years) and young (<20 years) male and female donors or starvation medium. (B) Treatment with sera from young female, young male and old female donors significantly increased the proliferation of hCSCs. (C) Orangu cell viability assay to measure the metabolism of hCSCs showed increased metabolism after serum treatment but no sex or age dependency. (D) SA-β-Galactosidase assay showed a decrease of senescent cells after plasma treatment compared to untreated cells. Mann-Whitney two-tailed, * p < 0.05 was considered significant.