Table A2.
Plasmids used in this study
| Plasmid | Genotype or relevant features | Reference or source |
|---|---|---|
| pBBR1MCS‐2‐lacZ | Broad‐host‐range reporter vector, a ND, promoterless lacZ gene(KanR) | Fried et al. (2012) b |
| p2cld‐I | pBBR1MCS‐2‐lacZ with 200 bp insert from 31 to 230 bp upstream start codon of cld | This work |
| p2cld‐II | Same as p4cld‐I except for 4 point mutations in predicted FNR box | This work |
| p2cld‐III | pBBR1MCS‐2‐lacZ with 151 bp insert from 80 to 230 bp upstream start codon of cld | This work |
| p2cld‐IV | pBBR1MCS‐2‐lacZ with 167 bp insert from 64 to 230 bp upstream start codon of cld | This work |
| pBR322 | Cloning vector, ColE1‐based replicon (TetR)(AmpR) | Thermo Fischer Scientific |
| pBR322(fnr Ec) | pBR322 with fnr gene (GeneID: 945908) and upstream sequences from Escherichia coli MG1655 | This work |
| pBR322(fnr Id) | pBR322 with fnr gene (img: 2510461017) and upstream sequences from Ideonella dechloratans | This work |
Abbreviations: AmpR, ampicillin‐resistant; KanR, kanamycin‐resistant; TetR, tetracycline‐resistant.
a
ND, the incompatibility group of pBBR1MCS vectors has not been defined; compatible with IncP, IncQ, and IncW group vectors, and with ColE1 and P15a based replicons.
b
pBBR1MCS‐2‐lacZ was kindly donated by Prof. K. Jung.