Table A3.

Primers used in this study. Restriction enzyme sites added to the primers are underlined

	Forward primer (5′–3′)		Reverse primer (5′–3′)	Product size (bp)
Amplification of upstream region of cld of Ideonella dechloratans
cld‐I	GGGCGAATTCGCGAGGTCGGCGTCAGT		GCGCAAGCTTGCTGGGCGGCTTGGTC	220
cld‐III	GGGCGAATTCGCGAGGTCGGCGTCAGT		GGCGCGTCGACTTTCTCCTTTTTTATGTGTTTGATT	172
cld‐IV	GGGCGAATTCGCGAGGTCGGCGTCAGT		GCGCGTCGACTGCACGAATCTTCGCATTTCTCCT	187
Mutagenesis of the putative FNR box with plasmid p4cld‐I as template
cld‐II	CCAAAAATATAGTGTTAAGTTTCGGAGAAACTCATTAATTAAAACAAACACATAAAAAAGGAGAAATGCGAAGATTC		GAATCTTCGCATTTCTCCTTTTTTATGTGTTTGTTTTAATTAATGAGTTTCTCCGAAACTTAACACTATATTTTTGG
Amplification of fnr genes from Escherichia coli or I. dechloratans
fnr E.c.	GGCAAGCTTAGGCGGAGTTCAGCGAAAAG		CGCGGATCCTGCCACCAATCCGTTGATGT	1,279
fnr I.d.	GGGCGAATTCCGTCGAATTGCCGCAACAAG		GCGCGGATCCCTCACAAGACCCCAGGGATG	1,053
qRT‐PCR
16SrRNA		CATCGGAACGTGCCCAGTAGTG	TGACATCGGCCGCTCCAATAG	119
fnr		CGCCTTCCTGATGAACCTGA	TAGCTGCCGATTTCTTCCCG	96