Figure 3.

Matched fecal samples aliquots stored in eNAT and frozen immediately to −80°C and stored in eNAT at ambient temperature do not have significantly different SDI, Chao1, global composition, and genus relative abundance. (a) A Bland–Altman plot of the Shannon diversity shows no proportional bias between sample preparation methods; (b) A Bland–Altman plot of the natural log‐transformed Chao1 index shows no proportional bias by sample preparation method; (c) The nonmetric multidimensional scaling distances of the Bray–Curtis Matrix show no difference in global microbiome composition by sample preparation method (PERMANOVA; p = .99); and (d) The median differences of relative abundances of the 20 most abundant bacterial genera are between 1.6% higher in eNAT frozen immediately to −80°C and 2.1% higher in eNAT stored at ambient temperature