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. 2020 Apr 21;8(2):192. doi: 10.3390/vaccines8020192

Figure 1.

Figure 1

Sensitivity and specificity of ELISA for p27 antibody detection. (a) The heat map shows the average optical density (OD) values of p27 monoclonal antibody (p27 mAb) from 3 independent ELISAs when different concentrations of p27 peptide, p27 mAb as well as alternative mAbs were used in the assay. Red and blue indicate strong and weak binding of p27 peptide coated on the plates and the corresponding mAb on the bottom of the figure, respectively. (b) The heat map shows the SEs (standard error of the mean) of OD values of p27 mAb from 3 independent ELISAs when different concentrations of p27 peptide, p27 mAb as well as alternative mAbs were used in the assay. Red and blue indicate high and low of SEs calculated, respectively. The heat map was made by GraphPad Prism version 8.3.0 for Windows, GraphPad Software, La Jolla California USA.