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. 2020 Apr 1;8(2):158. doi: 10.3390/vaccines8020158

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression and location of TgTH protein in T. gondii tachychites by immunofluorescence assay (×100 magnification). Panel A: T. gondii tachychites were probed by the serum of rats immunized by TgTH protein. (A1) Differential interference contrast (DIC). (A2) Immunofluorescence localization using Cy3. (A3) Dyeing nucleus by DAPI. (A4) DIC, Cy3, and DAPI combined. Panel B: Negative control, the tachychites were probed by the serum of normal rats without immunizing as the primary antibody. (B1) DIC. (B2) Cy3. (B3) DAPI. (B4) Combined.