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. 2020 Apr 1;8(2):158. doi: 10.3390/vaccines8020158

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The BALB/c mice were randomly divided into four groups of five mice each (n = 5). The animals in each group were immunized with recombinant protein TgTH mixed with Freund adjuvant (1:1), pET-32a protein mixed with Freund adjuvant (1:1), Freund adjuvant alone, and the rest of mice were blank controls. The levels of cytokine were determined by ELISA on 0, 2nd, 4th, and 6th weeks, and the results were expressed as the mean ± SD of concentration (pg/mL). As for the differences with statistical significance between groups in the identical time point, (***) referred to (p < 0.001). (A): IFN-γ. (B): IL-2. (C): IL-4. (D): IL-10. (E): IL-17.