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Infection intensity after secondary treatment. Five days after the primary treatment of naïve, injury, or infection with E. coli-K12, all the mosquitoes were infected with E. coli-GFP and the number of E. coli-GFP in their hemocoel was quantified 1 (A), 3 (B), or 7 (C) days later. Column heights mark the mean and whiskers denote the standard error of the mean (SEM). Circles mark the infection intensity of individual mosquitoes.
