Figure 1.

Sleeping Beauty transposon system for CAR T-cell engineering. Two-component DNA transposon-based gene delivery systems: Transposon plasmid carrying the gene of interest flanked by the ITRs and transposase expression plasmid; mechanism of ’cut and paste’ transposition of Sleeping Beauty for a stable genomic integration of the CAR; different transposase sources currently available: plasmid DNA, synthetic mRNA and synthetic recombinant protein; recombination of parental plasmids into minicircles and miniplasmids. ITR, inverted terminal repeat. Created with Biorender.com