Figure 2.
Induction of cell growth arrest by cold atmospheric plasma. (A) Representative images of the D-17 and DSN canine osteosarcoma cell lines treated with CAP. Scale bars, 100 μm. (B) Cell viability assay. D-17 and DSN cells were exposed to CAP at the indicated time. Cell viability was measured using CellTiter 96 AQueous One Solution. Error bars represent the mean ± S.E.M. of three replicates. (C). Measurement of de novo DNA synthesis. D-17 cells were stained with 5-ethynyl-2′-deoxyuridine (EdU), which stains the newly synthesized DNA, and the intensity was quantified by high-content screening technology. Green, EdU; and Blue, Hoechest 33342. Error bars represent the mean ± S.E.M. of three replicates. Magnification, 100X. * p < 0.05, ** p < 0.01, *** p < 0.001; N.S. indicates not significant.