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. 2020 Jun 26;21(12):4556. doi: 10.3390/ijms21124556

Figure 4.

Figure 4

Induction of apoptosis by cold atmospheric plasma (CAP) on canine osteosarcoma cells. (A) Microscopy analysis of chromatin condensation. Cells were stained with 4′,6-diamidino-2-phenylindole (DAPI) and observed under a fluorescence microscope. Apoptotic nuclei were detected in CAP-treated wells. Scale bars, 50 μm. (B) Measurement of change in mitochondrial membrane potential (Δ ψm). The cells were stained with MitoTracker, which is a stain for the mitochondrial membrane, and the intensity was measured by high-content screening technology. CAP-treated cells showed decreased Δ ψm compared to that in control. Error bars represent the mean ± S.E.M. of three replicates. Magnification, 100X. * p < 0.05, ** p < 0.01; N.S. indicates not significant. (C) Detection of Annexin V-positive cells. Cells were stained with Annexin V-FITC and propidium iodide and measured using flow cytometry. CAP induced an increase in Annexin V-positive cells in a time-dependent manner.