Figure 1.

Detection of α-Gal on glycoproteins of Toxocara canis and Ascaris suum by Western blot ((A,B), respectively) and indirect ELISA (C). Disaccharide Galα1-3Gal-HSA served as a positive control in both assays. The presence of α-Gal in the soluble proteins was assessed by a highly specific monoclonal anti-α-Gal antibody M86. Abbreviations: Tc-ExAD—crude extract of an adult female T. canis worm; Tc-ES—larval excretory/secretory antigens of T. canis (native antigen); As-ExAD—crude extract of an adult female A. suum worm; α-Gal—Galα1-3Gal-HSA antigen; OD—optical density.