Skip to main content
. 2020 May 27;9(6):1338. doi: 10.3390/cells9061338

Figure 3.

Figure 3

Induction of RELMα by OSM in whole lung IL-6–/– and STAT6–/– mice. (A) RELMα protein levels in BAL fluid from C57Bl/6 mice at day 2 or day 7 after AdDel70, AdOSM or AdIL-6 treatment, as assessed by ELISA (left panel). Total lung homogenates from separate C57Bl/6 mice 7 days post-infection were analyzed by Western blot for RELMα (right panel, 4 separate mice/group). (B) Total lung homogenates from wild-type and IL-6–/– C57Bl/6 mice 7 days post-infection were analyzed by ELISA (left panel) and Western blot (3 separate mice/group, right panel) for RELMα protein levels. (C) Total lung homogenates from wild-type and STAT6–/– C57Bl/6 mice 7 days post-infection were analyzed by ELISA (left panel) and Western blot (3 separate mice/group, right panel) for RELMα. Data for ELISAs (left panels) are expressed as mean ± SEM (n = 4–8 mice/group). Statistical significance was determined by one-way or two-way ANOVA with Tukey’s post hoc test (# p < 0.05 relative to controls at the indicated time point; * p < 0.05, *** p < 0.001, **** p < 0.0001 between indicated groups).