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Schematic diagram of generation genetically engineered pig for biomedical purposes. The different site-specific nucleases (ZFN, TALEN, CRISPR/Cas9) used for genome editing and two techniques (somatic nuclear transfer and microinjection) to produce genetically modified pigs are shown. Biomedical applications for which genetically engineered pigs are generated include modeling human diseases, production of pharmaceutical proteins, and xenotransplantation.
