Figure 2.

Composition of solubilized human liver extracellular matrix (HL-ECM). A proteomic analysis was performed on solubilized human liver extracellular matrix (HL-ECM), derived from 4 donors (154, 155, 158 and 159). (A) Schematic representation of the methodology applied to separately analyze peptide- and protein-enriched fractions. (B) String plot of the 52 proteins identified by mass spectrometry in the solubilized HL-ECM: each circle represents 1 protein; each line represents 1 identified protein–protein interactions in the String database; blue, red and purple filling indicate whether the protein was identified in the peptide, protein or both fractions; proteins were grouped in sets proposed in the Matrisome project. ACN: acetonitrile, ESI: electrospray ionization, LC: liquid chromatography, MS: mass spectrometry, TCA: trichloroacetic acid.