Table 1.
Composition of solubilized HL-ECM. Summary of the proteomic analysis of solubilized HL-ECM: gene and protein name, classification according to the Matrisome project, fraction where the protein was identified.
| Gene | Protein | Division | Function | Fraction |
|---|---|---|---|---|
| COL1A1 | Collagen I α1 chain | Core matrisome | Collagen | Both |
| COL1A2 | Collagen I α2 chain | |||
| COL2A1 | Collagen II α1 chain | |||
| COL3A1 | Collagen III α1 chain | |||
| COL4A2 | Collagen IV α2 chain | |||
| COL4A6 | Collagen IV α6 chain | |||
| COL5A1 | Collagen V α1 chain | |||
| COL6A3 | Collagen VI α3 chain | |||
| COL4A1 | Collagen IV α1 chain | Peptide | ||
| COL6A2 | Collagen VI α2 chain | |||
| COL8A1 | Collagen VIII α1 chain | |||
| COL8A2 | Collagen VIII α2 chain | |||
| EMILIN2 | EMILIN 2 | Core matrisome | ECM glycoprotein | Protein |
| ELN | Elastin | Both | ||
| FBN1 | Fibrillin 1 | |||
| EFEMP2 | EGF-containing fibulin-like ECM protein | Peptide | ||
| MFAP4 | Microfibril-associated glycoprotein 4 | |||
| ASPN | Asporin | Core matrisome | Proteoglycan | Peptide |
| BGN | Biglycan | |||
| DCN | Decorin | |||
| OGN | Mimecan | |||
| PRELP | Prolargin | |||
| VTN | Vitronectin | |||
| CTSD | Cathepsin D | Matrisome-associated | ECM regulator | Protein |
| CTSE | Cathepsin E | Both | ||
| SHH | Sonic hedgehog | Matrisome-associated | Secreted factor | Peptide |
| INHBE | Inhibin β E | |||
| NR3C2 | Mineralocorticoid receptor | Matrisome-unrelated | Membrane receptor | Protein |
| DEFA1 | Neutrophil defensin 1 | Matrisome-unrelated | Secreted protein | Protein |
| CHIA | Acidic mammalian chitinase | Both | ||
| APCS | Serum amyloid P-component | Peptide | ||
| HBB | Hemoglobin subunit β | |||
| DPAGT1 | DPAG phosphotransferase | Intracellular | Protein | |
| FOS | Proto-oncogene c-Fos | |||
| MCM10 | MCM 10 | |||
| MTA3 | Metastasis-associated protein 3 | |||
| NOC4L | Nucleolar complex protein 4 | |||
| NUP58 | Nucleoporin p58/p45 | |||
| NUTM2B | NUT family member 2B | |||
| PRDM14 | PR domain zinc finger protein 14 | |||
| XRCC5 | X-ray repair cross-complementing protein 5 | |||
| TPM4 | Tropomyosin α4 chain | Both | ||
| ARHGEF33 | Rho guanine nucleotide exchange factor 33 | Peptide | ||
| ATP9A | Probable phospholipid-transporting ATPase IIA | |||
| EEF1A | Elongation factor 1α | |||
| KMT2C | Histone-lysine N-methyltransferase 2C | |||
| HIST1H4 | Histone H4 | |||
| MAGEL2 | MAGE-like protein 2 | |||
| NCOA6 | Nuclear receptor coactivator 6 | |||
| RASA1 | Ras GTPase-activating protein 1 | |||
| TAF5 | Transcription initiation factor TFIID subunit 5 | |||
| VIM | Vimentin | |||
| KRT1 | Keratin type II cytoskeletal 1 | Probable contaminant | Protein | |
| KRT9 | Keratin type I cytoskeletal 9 | |||
| KRT10 | Keratin type I cytoskeletal 10 | |||
| ALB (B. taurus) | Serum albumin | Contaminant | Both | |
| PGA4 | Pepsin A4 | |||
| DNASE1 | Deoxyribonuclease 1 | Peptide | ||